TABLE 1.

Reaction states of E. cloacae MurA determined by crystallography

Complexa	MurA	Compounds provided	Ligands bound	Crystallization reservoir			PDB ID	Reference
				Buffer	Salt	Precipitant
I	WTb		UNAM	0.1 m Bis-Tris (pH 5.5)	0.2 m NH4OAc	25% PEG 3350	3SU9	This work
			+PEP-Cys
II	R120A	5 mm UNAG	UNAG	0.1 m HEPES (pH 7.5)		14% 2-Propanol, 20% PEG 4000	3SWA	This work
		+ 5 mm PEP	+PEP-Cys
III	D305A	5 mm UNAG	THI	0.01 m MES (pH 6.4)		10% PEG 20000	1Q3G	Ref. 5
		+ 5 mm PEP
IV	C115S	5 mm UNAG	EP-UNAG	0.01 m MES (pH 6.4)		10% PEG 20000	1RYW	Ref. 13
		+ 5 mm PEP	+Pi
V	WTc	2 mm UNAGal	EP-UNAGal	0.1 m HEPES (pH 7.5)	0.05 m MgCl2	30% PEG MME 550	3SWI	This work
		+ 2 mm PEP	+PEP-Cys
VI	WTb	2 mm UNAG	EP-UNAG	0.1 m Bis-Tris (pH 5.5)	0.2 m NH4OAc	25% PEG 3,350	3SWQ	This work
VII	WTc			0.1 m Bis-Tris (pH 5.5)	0.2 m NH4OAc	25% PEG 3350	1EJD, 1EJC, and 3SPB	Ref. 15 and this work
VIII	WTc	2 mm UNAG	UNAG	0.1 m Bis-Tris (pH 5.5)	0.2 m NH4OAc	25% PEG 3350	3UPK	This work
D-VII	C115Db			0.1 m Bis-Tris (pH 5.5)	0.2 m NH4OAc	25% PEG 3350	3V5V	This work
D-VIII	C115Db	2.5 mm UNAG	UNAG	0.1 m Bis-Tris (pH 5.5)	0.2 m NH4OAc	25% PEG 3350	3V4T	This work

^a Complex identifiers are the same as in Figs. 7 and 8.

^b Untreated.

^c Treated with 50 mm phosphate buffer before crystallization.