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. 2012 Feb 21;287(16):12835–12847. doi: 10.1074/jbc.M112.340109

FIGURE 1.

FIGURE 1.

Expression of VapC-mt4 leads to growth arrest. A, growth profile for VapB-mt4 and VapC-mt4 uninduced (□), VapB-mt4 and VapC-mt4 induced (♦), or pINIII and VapC-mt4 induced (●) in E. coli BW25113Δ6 containing either pINIII or pINIII-VapB-mt4 and pBAD33-VapC-mt4 grown at 37 °C in M9 minimal medium containing 0.1% glycerol. Both 0.2% arabinose and 1 mm isopropyl 1-thio-β-d-galactopyranoside were added to the induced samples. An empty pINIII vector was used to assess VapC-mt4 toxicity because leaky expression of VapB-mt4 from pINIII-VapB-mt4 abrogated VapC-mt4 toxicity. B, growth profile for pMC1s (□), pMC1s-VapBC-mt4 (♦), or pMC1s-VapC-mt4 (●) in M. tuberculosis H37Rv grown at 37 °C in 7H9-TW80-ADN medium containing 30% spent culture supernatant and 200 ng/ml anhydrotetracycline. Cultures were inoculated with single colonies. Additional anhydrotetracycline was added every 48 h to maintain the anhydrotetracycline concentration between 12.5 and 200 ng/ml. Data points represent the average of three independent experiments; error bars represent the S.D.