FIGURE 3.

VapC-mt4-mediated sequence-specific RNA cleavage. Primer extension analysis for tufA (A and B), ompA (C), and lpp (D) mRNAs extracted from E. coli BW25113Δ6 cells following induction of VapC-mt4. Numbers indicate the time (min) after VapC-mt4 induction. Cleavage sites/products are indicated by black arrowheads on the right side of gels and in the relevant RNA sequences/corresponding amino acid sequences below each gel. Lanes G, A, T, and C correspond to DNA sequencing ladders prepared using the same primers used in the primer extension reactions. E, alignment of sequences containing VapC-mt4 cleavage sites. The ACGC consensus sequence is in bold.