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MT6-MMP cleaves cystatin C, IGFBP-7, galectin-1, and SPARC. Candidate substrates (1 μg) cystatin C (A), IGFBP-7 (B), galectin-1 (C), and SPARC (D) were incubated in a 10-μl reaction at an enzyme to substrate of 1:10 (w/w) with sMT6-MMPΔF for 16 h at 37 °C. Cleavage assay products were visualized under reducing conditions by silver-stained SDS-PAGE. The arrow indicates full-length protein; the broken arrow indicates cleavage product in the presence of MT6-MMP.
