. 2012 Feb 24;287(16):13382–13395. doi: 10.1074/jbc.M111.314179

TABLE 1.

Highest confidence substrates of MT6-MMP

Substrates were identified by the same peptide in biological replicates following HFL secretome treatment with sMT6-MMPΔF (114 label) or sMT6-MMP(E234A) (115 label) having a 114/115 ratio of <0.71 or >1.4 based upon a standard deviation of 0.24. Thus, these peptides represent the C-terminal cleavage product after cleavage by MT6-MMP.

Protein	Ratio 114/115	Peptide
Actin	0.64	²⁰GFAGDDAPR
	2.22	²⁹AVFPSIVGR
	11.14	¹⁰⁵LTEAPLNPKANR
	4.41	²⁹⁸VLSGGTTMYPGIADR
	4.53	⁶LVVDNGSGMCKAGFAGDDAPR^a
	26.19	⁴³VMVGMGQKDSYVGDEAQSKR^b
	21.62	⁵²SYVGDEAQSKR^b
Collagen type I α-1 chain	10.29	¹⁰⁰¹GPPGLAGPPGESGR
	31.73	⁹⁷⁷GPSGEPGKQGPSGASGER^b
	10.98	¹⁰⁰⁴GLAGPPGESGR^b
	36.17	¹⁰⁰⁵LAGPPGESGR^b
	42.57	¹⁰⁰⁶AGPPGESGR^b
	12.67	¹⁰⁵⁰APGAPGPVGPAGKSGDR^b
	12.27	¹⁰⁵²GAPGPVGPAGKSGDR^b
	0.36	¹⁰⁷⁵AGPVGPVGAR^b
	6.55	¹²³⁷SLSQQIENIR^b
	23.76	¹²³⁹SQQIENIR^b
Collagen type I α-2 chain	1.42	⁸⁶⁸GAPGILGLPGSR
	2.33	³³³VGAAGATGAR
	4.68	¹¹⁴¹SLNNQIETLLTPEGSR
	17.94	¹¹³FQGPAGEPGEPGQTGPAGAR^b
	14.84	¹¹⁵GPAGEPGEPGQTGPAGAR^b
	0.65	¹²¹GEPGQTGPAGAR^a
	20.15	³³¹GPVGAAGATGAR^b
	48.93	³⁷⁰GPPGPSGEEGKR^b
	1.48	³⁸⁵GEAGSAGPPGPPGLR^a
	43.99	⁵⁵⁹GPSGPAGEVGKPGER^b
	7.94	⁷⁸⁴GMTGFPGAAGR^b
	23.30	⁸⁰⁸GPPGPAGKEGLR^b
	3.10	⁸⁶⁸GAPGILGLPGSR^b
	24.51	⁹⁶⁷GPVGPAGKHGNR^b
	38.25	¹¹⁰³VSGGGYDFGYDGDFYR^b
	10.94	¹¹⁰⁹DFGYDGDFYR^b
	14.89	¹¹¹⁰FGYDGDFYR^b
	29.73	¹¹¹¹GYDGDFYR^b
	27.86	¹¹¹²YDGDFYR^b
	20.83	¹¹⁴⁴NQIETLLTPEGSR^b
	39.22	¹¹⁴²LNNQIETLLTPEGSR^b
	25.98	¹¹⁴³NNQIETLLTPEGSR^b
	42.37	¹¹⁴⁶IETLLTPEGSR^b
	22.07	¹¹⁴⁹LLTPEGSR^b
Cystatin C	5.54	³⁵LVGGPMDASVEEEGVRR
	26.71	³⁵LVGGPMDASVEEEGVR
	26.35	³⁶VGGPMDASVEEEGVR^b
	7.48	³⁷GGPMDASVEEEGVR^b
	31.22	⁴²ASVEEEGVR^b
IGFBP-5	0.63	¹⁶⁴KFVGGAENTAHPR
	2.97	²¹³AVYLPNCDR
	0.18	¹⁶⁵FVGGAENTAHPR^b
	0.29	¹⁶⁶VGGAENTAHPR^a
IGFBP-7	1.46	⁹⁸AGAAAGGPGVSGVCVCKSR
	2.42	⁹⁸AGAAAGGPGVSGVCVCKSR^b
	6.72	⁹⁹GAAAGGPGVSGVCVCKSR^b
	32.03	¹⁰⁰AAAGGPGVSGVCVCKSR^b
MT6-MMP	8.38	³¹⁸EGNFDAIANIR
	36.59	²⁹VSLGVDWLTR^a
	8.67	²⁵⁴FYQGPVGDPDKYR^a
	4.41	²⁵⁵YQGPVGDPDKYR^a
	23.62	³⁵⁹FWEGLPAQVR^b
	8.73	³⁸⁶SGPQFWVFQDR^a
	17.25	⁴⁷³GDTYFFKGAHYWR^a
Transgelin	0.66	²ANKGPSYGMSR
Tropomyosin α-4 chain	3.56	¹³³LVILEGELER
	0.68	¹⁶ALQQQADEAEDR^a
	19.62	¹¹⁵AKHIAEEADR^b
	15.24	¹³⁴VILEGELER^b
Vimentin	0.58	⁵⁵SSPGGVYATR
	2.05	⁸⁷SLADAINTEFKNTR
	1.55	⁸⁸LADAINTEFKNTR
	0.30	⁵⁴ASSPGGVYATR^b
	0.65	⁹¹AINTEFKNTR^a
	35.77	¹¹⁴FANYIDKVR^b
	0.39	²⁵⁸VDVSKPDLTAALR^b
	8.83	²⁹⁵FADLSEAANR^b
	2.69	²⁹⁶ADLSEAANR^b
	4.21	³³⁰VDALKGTNESLER^b
YIPF3	0.71	³²¹DIPAMLPAAR
26 S protease regulatory subunit 8	1.82	²ALDGPEQMELEEGKAGSGLR

^a Additional peptide identification in TAILS1.

^b Additional peptide identification in TAILS2.