. 2012 Feb 24;287(16):13382–13395. doi: 10.1074/jbc.M111.314179

TABLE 2.

High confidence substrates of MT6-MMP

Substrates were identified by ≥2 spectra, including multiple charge states of modifications of the peptide, following HFL secretome treatment with sMT6-MMPΔF (114 label) or sMT6-MMP(E234A) (115 label) having a 114/115 ratio of <0.68 or >1.46 based upon a standard deviation of 0.27.

Protein	Ratio 114/115	Peptide	No. of spectra
α-Actinin-1	24.56	¹³MQPEEDWDR^a	2
Collagen type II α-1 chain	43.76	⁵⁷⁶GPAGPAGER^a	2
	8.59	⁸²⁵GATGFPGAAGR^a	2
Collagen type IV α-2 chain	6.84	⁷⁶GLQGFPGLQGR^a	1
	20.66	⁴⁶¹FPGLPGSPGAR^a	1
	32.24	⁶⁵¹GPAGTPGQIDCDTDVKR^a	1
	5.81	⁶⁵⁵TPGQIDCDTDVKR^a	1
Collagen type VI α-1 chain	1.71	⁴⁴³GDPGEAGPQGDQGR^b	1
	16.85	⁴⁹⁷GPPGDPGLMGER^a	2
	7.81	⁵⁰⁰GDPGLMGER^a	1
Collagen type VI α-3 chain	2.96	³¹⁰⁶LTETDICKLPKDEGTCR^a	1
Dickkopf-related protein 3	33.89	¹³¹TVITSVGDEEGR^a	2
	15.46	⁵⁴VEELMEDTQHKLR^a	1
	51.66	²⁵²ITWELEPDGALDR^a	1
Extracellular matrix protein 1	0.15	²⁰ASEGGFTATGQR^a	3
Fibrillin-1	0.37	²⁵ADANLEAGNVKETR^a	2
Latent transforming growth factor β-binding protein 2	1.48	¹⁷²⁹FEGLQAEECGILNGCENGR^b	1
	5.36	²⁴⁹SSAAGEGTLAR^a	1
MMP-1	4.98	³²⁸LEAAYEFADR^b	2
	7.30	³⁶⁴IYSSFGFPR^b	1
Peptidyl-prolyl cis-trans isomerase A	7.88	⁹DIAVDGEPLGR^a	3
	8.52	⁸FDIAVDGEPLGR^a	1
Procollagen C-endopeptidase enhancer 1	37.52	³⁰⁹SPSAPDAPTCPKQCR^a	2
Protein-lysine 6-oxidase	0.61	⁵⁹SLGSQYQPQR^b	2
	4.75	⁵⁷LLSLGSQYQPQR^b	2
	0.39	²²APPAAGQQQPPR^a	1
	2.12	⁵⁸LSLGSQYQPQR^b	1
Serpin H1	0.23	¹³⁹SVSFADDFVR^b	2
Sulfhydryl oxidase 1	42.01	⁵⁶⁵AMGALELESR^a	7
	32.69	⁵⁶⁶MGALELESR^a	2
Stromal cell-derived factor 4	12.27	⁸⁴GKDLGGFDEDAEPR^a	1
	7.94	⁸⁸GGFDEDAEPR^a	1
Syndecan-4	7.00	²⁴TEVIDPQDLLEGR^a	2
	7.49	²⁶VIDPQDLLEGR^a	1
Tropomyosin 3	6.84	¹⁷⁰LVIIEGDLER^a	1
	38.06	¹⁷¹VIIEGDLER^a	2
Tropomyosin β chain	41.62	²²AEQAEADKKQAEDR^a	2
	48.86	²⁴QAEADKKQAEDR^a	1
	17.45	⁷⁴AEKKATDAEADVASLNR^a	1
	23.20	¹⁵¹AKHIAEDSDR^a	1

^a Additional peptide identification in TAILS2.

^b Additional peptide identification in TAILS1.