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β-Arrestin2 bimolecular fluorescence complementation assay. (SH2-D9)MOP-VC cells were transiently transfected with β-arrestin2-VN. 48 h post-transfection, living cells were incubated at room temperature in KRH buffer alone (Control) or containing 1 μm DAMGO or 1DMe and observed for 30 to 60 min under a Zeiss LSM 510 inverted confocal microscope with ×63 objective at λexc 488 nm. Representative images of the 30-min time point are shown.
