FIGURE 2.
miR378 is a stress inducible microRNA in rat neonatal cardiomyocytes. A, developmental and age-related expression of miR-378 by Northern blotting. B, expression of miR-378 following treatment of neonatal cardiomyocytes with various stressors for the indicated times and durations. Lower panel shows the expression of a non-related microRNA, miR-208, in the same membrane after stripping and hybridization with radiolabeled miR-208. C, quantification of miR-378 normalized to miR-208a, each bar is the mean ± S.D. of a minimum of 3 independent experiments. D, real-time PCR analysis of Pgc1β mRNA in the same samples as in B. Relative expression was calculated by the ΔΔCT method using β-actin as a reference gene. Asterisks mark the statistical significance (p < 0.05). NS, non-significant.