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IGF1R as a predicted target of miR-378. A, sequence alignment of IGF1R 3′ UTR with the miR-378 seed sequence (highlighted in the box) showing the position of the predicted binding site and species conservation. B, Western blot analysis of IGF1R in the fetal heart and after birth (50 μg of protein/lane). C, inverse expression pattern of miR-378 and IGF1R protein levels in cardiac tissues at the indicated development time. Blots are representative of a minimum of 3.
