FIGURE 5.

Inhibition of IGF1 but not PE-induced signaling and augmentation of PQ-401 effect on pAkt by miR-378. A, Western analysis showing pAkt (Thr³⁰⁸) and total Akt in neonatal rat cardiomyocytes following transfection with mimic control or 378-mimic for 48 h, cells were then serum starved overnight and treated with IGF1 (10 nm) for 15 min. B, same cell lysates as in A analyzed for pERK and total ERK2. C, cells were prepared as in A, but treated with phenylephrine (50 μm) for 5 min and analyzed for pERK and total ERK. D, cells were prepared as in A, but treated with PQ-401 (10 μm) 90 min prior to stimulation with IGF1. All results are representative of at least three independent experiments. p < 0.05, Significant when compared with the non-treated group (*) or when compared with the corresponding treated mimic control group (#).