FIGURE 7.

miR-378 knockdown enhances IGF1R expression and IGF1-induced AKT activation. A, Northern blot showing knockdown of miR-378 in cardiac myocytes 48 h after transfection with either scramble control or 378-anti-miR, U6 represents the loading control on the same membrane. B, Western analysis of IGF1R 72 h after transfection of cardiomyocytes with scramble or increasing amounts of 378-anti-miR. C, expression of pAkt and total Akt in cardiomyocytes transfected with scramble control or 378-anti-miR. After a 48-h transfection, cells were incubated in serum-free media overnight and then treated with IGF1 for 15 min. D, cells were prepared as in C and treated with the IGF1R inhibitor PQ-401 (10 μm) 90 min prior to IGF1 treatment. p < 0.05, *, compared with non-PQ treated scramble control; #, compared with the PQ-401-treated scramble control (n = 2).