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. 2012 Jan 31;287(16):12679–12690. doi: 10.1074/jbc.M111.336180

FIGURE 2.

FIGURE 2.

Abcb6 is the sole ATP-dependent mitochondrial transporter of coporphyrin III. A, comparison of CP import in wild-type and Abcb6−/− mice, showing ATP-dependent transport (ATP), non-ATP-dependent transport (A-N-P), and total transport (ATP transport with non-ATP transport subtracted). B, import kinetics of CP in mitochondria isolated from mouse liver. C, comparison of rate of CP import into untreated and HMBA-treated MEL cells. D, immunoblot of mitochondrial lysates shows that non-ATP-dependent carriers are not up-regulated in Abcb6−/− mitochondria and that Abcb6 is maximally up-regulated in HMBA-treated MEL cells. E, time course of heme concentration in untreated and HMBA-treated MEL cells, as measured by HPLC and normalized by cell number. F, HPLC analysis of intracellular porphyrins in untreated and HMBA-treated MEL cells. VDAC, voltage-dependent anion channel; PBR, peripheral benzodiazepine receptor; OGC, 2-oxoglutarate carrier; ANT, adenine nucleotide translocator; Cpx III, coproporphyrin III.