Figure 1. Profiling of SPM in E. coli infections.

Mice were inoculated with E. coli at (a) 10⁵ or (b) 10⁷ CFU by intraperitoneal injection, and peritoneal leukocytes enumerated. Results are expressed as mean±s.e.m., n=4-6. See Methods for calculation of resolution indices. *p<0.05, **p<0.01, vs. 24h; ^#p<0.05, ^##p<0.01, ^###p<0.001^, vs. 48h. (c) Time course of SPM and related pathway markers. Results are expressed as mean±s.e.m. of n=5 separate time courses. *p<0.05, **p<0.01, self-resolving (10⁵ CFU) vs. higher titer (10⁷ CFU) E. coli exudates. (d) Representative MRM chromatograph of eicosanoid, resolvin and protectin pathway products from naive germ-free mice. Each LM was identified based on published LC-MS-MS³⁰ (see Table S1). (e) SPM and pathway markers in colons of germ-free and conventional mice; representative of 3 mice. (f) Representative MS-MS of RvD1 (from germ-free mice) and RvD5 (from E. coli-infected mice); a=[M-H] (parent ion).