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. 2012 Apr 5;109(17):6584–6589. doi: 10.1073/pnas.1113271109

Fig. 5.

Fig. 5.

The HRG/PI3K/NF-κB axis controls mammosphere formation of primary tumor cells derived from breast cancer patients. (A) Representative images of primary cultures of mammospheres incubated with 20 ng/mL HRG, 5 μg/mL DHMEQ and 5 μM LY294002. Scale bar = 100 μm. (B) Primary breast cancer cells obtained from specimens of invasive ductal carcinoma (IDC1, 2, and 3) or invasive lobular carcinoma (ILC1) were treated as indicated in A, and the percentage of mammosphere-forming cells was determined (data are mean ± SD; n = 4). (C) Cells from IDC1 were incubated with 100 ng/mL HRG for 10 min, and protein expression levels were determined by immunoblotting. (D) Cells from IDC1 expressing the indicated lentiviral vectors were incubated with 20 ng/mL HRG or with EGF/bFGF/B27, and the percentage of mammosphere-forming cells was determined (data are mean ± SD; n = 4, **P < 0.01, relative to the values in the respective controls).