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Schematic representation of (A) nanofabricated double-tethered DNA curtain assay, (B) λ phage DNA bearing the two native 1xKOPS sites and the engineered 3xKOPS site, and (C) the covalently linked FtsK trimer with an N-terminal biotin tag. (D) Images of a double-tethered YOYO1-stained DNA curtain (green) bound by QD-tagged FtsK (magenta). The DNA, FtsK, and corresponding overlay are shown at Top, Middle, and Bottom, respectively.
