Fig. 1.

HSP2 transcription in vitro. (A) Schematic of mtDNA promoters: LSP, HSP1 and HSP2, interpromoter region, TRE for LSP and HSP1, and nearby genes. This region of mtDNA was used to produce LONG and SHORT templates capable of producing the indicated runoff transcripts. (B) Runoff transcription from the LONG or SHORT templates and indicated factors. All experimental details for this experiment and subsequent experiments are provided in SI Materials and Methods. M designates size markers. (C) Sequence of DNA oligonucleotide representing HSP2; the proposed TSS is indicated by +1. (D) Runoff transcription from the HSP2-1 template and indicated factors.