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Repression of HSP2 transcription by TFAM orthologs in vitro. (A) Alignment of carboxyl-terminal sequences of TFAMs from human, mouse, and bovine species. (B) TFAMs from mouse and bovine inhibit runoff transcription from the HSP2-1 template by POLRMT-TFB2M. (C) EMSA of HSP2-1 template over a range of mouse and bovine TFAM concentrations shows formation of four complexes (I–IV), with complex I present from 0.5 nM as observed for human TFAM in Fig. 3C.
