Fig. 1.

Evaluation of CD47 expression in LMS versus benign leiomyoma. (A) Gene-expression profiling on 51 LMS and on 19 benign leiomyoma samples revealed that the transcript levels of CD47, as well as TAM markers CD68 and CD163, were significantly higher on LMS versus their benign counterparts. P values were calculated using Student t test. (B) Immunofluorescence staining with anti-CD47 (Upper) or isotype control (Lower) antibodies on 16 LMS, three leiomyoma, and three normal muscle samples revealed that CD47 protein was more highly expressed on LMS than on benign or normal tissues. Representative images are shown and additional images are available Fig. S1 (see also Table S1). Magnification, 200×.