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Transferrin uptake and endocytosis of endogenous transferrin receptors (TfRs) is inhibited during mitosis despite abundant surface TfR. Summary bar charts of flow cytometry experiments to test the effect of cell cycle stage on uptake of Alexa488-conjugated transferrin (A), the ratio of internalized to surface labeled TfR (B), and the abundance of TfR at the cell surface (C). Data are mean ± SEM of at least three independent experiments. *P < 0.05, **P < 0.01.
