Fig. 4.

VCAM–MPIO binding and tumor detection in the MDA231BR model. (A) Selected T₂*-weighted images from a 3D dataset (coordinates relative to Bregma) at day 21 after intracardiac injection of MDA231BR cells. Intense focal hypointense areas (black) correspond to VCAM–MPIO binding (Scale bar: 1 mm.) (B and C) Colocalization of the MRI hypointense signals (arrows) with VCAM-1 expression (brown) and metastases (arrows). C1–C4 show higher magnification photomicrographs of the four tumor colonies highlighted in C. (Scale bars: B, 1 mm; C, 200 μm; C1–C4, 50 μm.) (D) 3D reconstruction shows the spatial distribution of VCAM–MPIO binding (red).