Fig. 3.
(A and B) Luciferase reporter assays of rs62527607 with cotransfected transcription factor RUNX1. Luciferase reporter constructs containing rs62527607 were transfected in triplicate into murine neuroblastoma cells (Neuro2a) and cotransfected with pIRES2-RUNX1-EGFP or empty expression construct as control. Luciferase expression levels were normalized using a cotransfected Renilla construct (±SD). Addition of the RUNX1 expression construct to the pGL4.11 system increased the activity of rs62527607[T] but not rs62527607[G] (T allele; P = 0.02; G allele: P = 0.27) (A). Using the minimal promoter system (pGL4.24) luciferase activity of both rs62527607 alleles increased (T allele: P = 6.13 × 10−4; G allele: P = 8.40 × 10−3), with rs62527607[T] being the more responsive allele (pGL4.11: 1.19-fold vs. 1.03-fold increase; pGL4.24: 1.67-fold vs. 1.35-fold increase) (B).