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Electrophoretic mobility shift assay (EMSA) of rs62527607[T] vs. rs62527607[G]. Nuclear extract of KG1a cells (NE) was added to a 20-bp biotin-labeled sequence (probe) containing either the T or G allele. For supershifting, RUNX1 antibody (AB) was added. RUNX1 bound to a greater extent to the sequence containing rs62527607[T], and the addition of the RUNX1-AB disrupted the binding.
