Table 2.

Preservative effectiveness testing for epoprostenol AM stored at 25°C (reduction in count^a)

Time (hours)	Sterile water for injection						Sterile saline (sodium chloride 0.9%) for injection
	S. aureus	P. aeruginosa	E. coli	C. albicans	A. niger	C. sporogenes	S. aureus	P. aeruginosa	E. coli	C. albicans	A. niger	C. sporogenes
	3000 ng/mL
12	0.48 log	>0.60 log	0.55 log	−0.25 log	−0.18 log	>0.30 log	>0.60 log	>0.48 log	>0.90 log	0.18 log	−0.40 log	0.30 log
24	>0.48 log	>0.60 log	0.85 log	−0.10 log	−0.40 log	0.30 log	>0.60 log	>0.48 log	>0.90 log	0.18 log	−0.40 log	>0.30 log
36	>0.48 log	>0.60 log	0.85 log	0.60 log	0.00 log	0.30 log	>0.60 log	>0.48 log	>0.90 log	0.48 log	0.30 log	−0.18 log
48	>0.48 log	>0.60 log	0.85 log	0.00 log	−0.30 log	0.30 log	>0.60 log	>0.48 log	>0.90 log	0.48 log	0.30 log	0.30 log
	6000 ng/mL
12	>0.48 log	>0.60 log	0.85 log	0.12 log	−0.18 log	>0.30 log	>0.60 log	>0.48 log	>0.90 log	0.18 log	0.30 log	−0.18 log
24	>0.48 log	>0.60 log	>0.85 log	0.12 log	0.30 log	0.00 log	>0.60 log	>0.48 log	>0.90 log	0.18 log	0.30 log	0.30 log
36	>0.48 log	>0.60 log	>0.85 log	>0.60 log	0.30 log	0.30 log	>0.60 log	>0.48 log	>0.90 log	0.18 log	>0.30 log	0.30 log
48	>0.48 log	>0.60 log	>0.85 log	0.12 log	0.00 log	>0.30 log	>0.60 log	>0.48 log	>0.90 log	0.48 log	>0.30 log	0.30 log
	15,000 ng/mL
24	0.3 log	1.3 log	0.6 log	0.1 log	0.00 log	nd	>0.9 log	>1 log	>1 log	0.2 log	0.00 log	nd
48	1.0 log	>1 log	1.0 log	0.7 log	0.00 log	nd	>1 log	>1 log	>1 log	0.2 log	0.00 log	nd
72	>1 log	>1 log	>1 log	>1 log	0.00 log	nd	>1 log	>1 log	>1 log	0.5 log	0.00 log	nd
96	>1 log	>1 log	>1 log	>1 log	0.00 log	nd	>1 log	>1 log	>1 log	0.8 log	0.00 log	nd
	30,000 ng/mL
24	>0.48 log	>0.60 log	>0.85 log	>0.60 log	0.00 log	0.30 log	>0.60 log	>0.48 log	>0.90 log	0.48 log	0.00 log	0.30 log
48	>0.48 log	>0.60 log	>0.85 log	>0.60 log	0.00 log	0.30 log	>0.60 log	>0.48 log	>0.90 log	>0.48 log	0.30 log	>0.30 log
72	>0.48 log	>0.60 log	>0.85 log	>0.60 log	−0.30 log	>0.30 log	>0.60 log	>0.48 log	>0.90 log	>0.48 log	0.00 log	>0.30 log
96	>0.48 log	>0.60 log	>0.85 log	>0.60 log	−0.18 log	>0.30 log	>0.60 log	>0.48 log	>0.90 log	>0.48 log	−0.18 log	>0.30 log

Notes:

^aReduction in the number of CFU relative to the initial inoculation. Negative numbers indicate an increase in CFU over the initial inoculum. ‘No increase’ was defined as no increase in CFU > 0.5 log₁₀.

Abbreviations: AM, arginine and mannitol; CFU, colony-forming units; nd, not done.