FIGURE 1.

BpGH117 shows neoagarobiose hydrolase activity. A, neoagaro-oligosaccharides, here represented by neoagarotetraose, as substrates for β-agarases and α-agarases (i.e. GH117). The LA residue at the non-reducing end is degraded to LA and G-LA-G by BpGH117. B, TLC analysis of neoagarose oligosaccharide degradation by BpGH117. Lanes 1, 2, and 3 show the untreated standards, whereas lanes 4, 5, and 6 show the products resulting from treatment of the di-, tetra-, and hexasaccharide standards with BpGH117.