TABLE 1.

Oligonucleotide primers used for gene amplification and mutagenesis

Primer name	Sequence
BpGH117F	5′-CATATGGCTAGCAATACACAGACTATTGCCG-3′
BpGH117R	5′-GTGTGTCTCGAGTTATTTACGTTCTTTTGATTCA-3′
D90NF	5′-GTAGTAAGAAGAAACCCGAGTGCAATGCTG-3′
D90NR	5′-CAGCATTGCACTCGGGTTTCTTCTTACTAC-3′
E167QF	5′-CTGTTTTCACTCCTAAGGTAATGGAATGG-3′
E167QR	5′-CCATTCCATTACCTTAGGAGTGAAAACAG-3′
D245NF	5′-CATAAGGTACACAACCCGTGTATAATACC-3′
D245NR	5′-GGTATTATACACGGGTTGTGTACCTTATG-3′
E303QF	5′-CAAACAGTGGGCATCAAGTCTGCGTCTGG-3′
E303QR	5′-CCAGACGCAGACTTGATGCCCACTGTTTG-3′
H302QF	5′-CAAACAGTGGGCAAGAAGTCTGCGTCTGG-3′
H302QR	5′-CCAGACGCAGACTTCTTGCCCACTGTTTG-3′
H302EF	5′-CAAACAGTGGGGAAGAAGTCTGCGTCTGG-3′
H302ER	5′-CCAGACGCAGACTTCTTCCCCACTGTTTG-3′