FIGURE 5.

Nucleotide incorporation opposite an abasic site analog F. A, structure of nucleotide analog dNITP. B, partial primer/template sequence used in primer extension experiments. C, single nucleotide (nt) incorporation of KlenTaq wild type opposite F for 1, 10, or 60 min, respectively. The respective dNTP is indicated. Transient kinetic analysis of nucleotide incorporation opposite T/F by KlenTaq wild type was performed. *, kinetic data were first reported in Ref. 19. D, single nucleotide incorporation of KlenTaq Y671W mutant opposite F for 10, 60, or 120 min, respectively. The respective dNTP is indicated. Transient kinetic analysis was performed as in C for KlenTaq Y671W.