FIGURE 6.
Geometry fit to active site. A, active site assembly in the presence of an abasic site analog F. The nascent base pair of Tyr-671 and ddGTP (KlenTaqF-G-I) is depicted. The surface of the surrounding active site residues is shown in gray. B, same as in A for KlenTaqF-G-II. C, active site assembly in the case of an undamaged template showing the nascent base pair of dC and ddGTP (KlenTaqC-G). D, same as in A for KlenTaqF-T. E, same as in A for KlenTaqF-A. F, same as in C for KlenTaqF-NI showing the nascent base pair of F and dNITP. A–F, the arrows indicate the positioning of the α-phosphate and the 3′-primer terminus, respectively.