FIGURE 1.
Up-regulation of BLT2 expression in aggressive ovarian cancer cells. A, the abundances of BLT1 and BLT2 mRNAs in CAOV-3, OVCAR-3, and SKOV-3 ovarian cancer cells were determined by semiquantitative RT-PCR (left panel) and real time PCR analysis (middle and right panels). B, the expression of BLT2 on cell surface was determined by FACS analysis. MFI, mean fluorescence intensity. †, p < 0.01; ‡, p < 0.001. C, CAOV-3 (7 × 105), OVCAR-3 (7 × 105), and SKOV-3 (5 × 105) cells were cultured in 100-mm dishes for 48 h, after which the amounts of LTB4 and 12(S)-HETE in the culture supernatants were measured by ELISAs (*, p < 0.05). D, immunoblot analysis of 5-LO, 12-LO, and FLAP in ovarian cancer cell lines. E, cells (4 × 104) were seeded on Matrigel inserts of an invasion chamber in medium containing 0.5% FBS, and medium containing 10% FBS was placed in the lower compartment of the chamber. After incubation for 48 h, the number of cells that had invaded through the Matrigel matrix was determined. All of the quantitative data are the means ± S.D. from three independent experiments. †, p < 0.01.