FIGURE 3.

LDLR overexpression increases the cellular degradation of Aβ by primary astrocytes. A, schematic diagram of the experiments used to measure degradation of ¹²⁵I-Aβ by primary astrocytes. ¹²⁵I-Aβ was added to primary astrocytes from either WT or LDLR Tg mice at the indicated time points. After each time point, media was collected and a TCA precipitation was performed to detect degraded Aβ. B, the supernatant (sup) and pellet counts/min are plotted as a function of time. Representative data from one experiment is shown. Experiment was repeated three times with similar results. C, degraded Aβ was quantified by calculating the percent of Aβ degraded as a percent of the total intact Aβ added. Mean ± S.E., * denotes p < 0.05, ** denotes p < 0.01. D, to measure the ability of astrocyte-conditioned media to degrade Aβ, media was collected from either WT or LDLR Tg primary astrocytes. ¹²⁵I-Aβ was then added to the astrocyte-conditioned medium at the indicated time points and a TCA precipitation was performed. The supernatant (sup) and pellet counts/min are plotted as a function of time. Representative data from one experiment is shown. The experiment was repeated two times with similar results. E, degraded Aβ was quantified by calculating the percent of Aβ degraded as a percent of the total intact Aβ added. Mean ± S.E., * denotes p < 0.05, n.s., not significant.