TABLE 4.

Activity of AcGlcA79A wild type and E45D mutant for PNP-β-GlcA and MeGlcA-β-1,6-Gal₂

The assay using MeGlcA-β-1,6-Gal₂ was performed in duplicate. Results are means ± S.E.

	kcat/Km		Ratioa (MeGlcA/GlcA)	Relative to WT
	PNP-β-GlcA	MeGlcA-β-1,6-Gal2
	mm−1·s−1
Wild type	2,303 ± 355	7.7 × 10−3 ± 1.2 × 10−3	3.0 × 10−6	1
E45D	7.5 ± 1.7	1.1 × 10−3 ± 0.1 × 10−3	1.3 × 10−4	43

^a (k_cat/K_m for MeGlcA-β-1,6-Gal₂)/(k_cat/K_m for PNP-β-GlcA).