FIGURE 3.
Signaling events elicited in response to apoptotic targets are long lived. Serum-starved BU.MPT responder cells were exposed to vehicle or apoptotic targets (Apo) at a target/responder cell ratio of 1:1 for 15 min. The source of apoptotic targets was staurosporine-treated BU.MPT cells. At the indicated times following stimulation (t = 0), non-adherent cells were removed by washing, and responder cell lysates were probed with anti-active Akt and anti-active p90RSK antibodies as shown. Equal loading was confirmed by Ponceau S staining of blotted proteins as well as by probing for total Akt.