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. 2012 Mar 1;287(17):13674–13685. doi: 10.1074/jbc.M111.320705

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — The conserved C-terminal domain of Sprouty2 is essential for GRIN interaction. A, schematic diagram of the Sprouty2. B, constructs used in these studies and summary of results. ΔC1 to ΔC5 mSprouty2 truncations were tagged with an HA epitope. Mouse Sprouty2 truncations ΔC6 and ΔC7 were made into fusion proteins with the pDsRed. C, Sprouty2 regions essential for GRIN2 binding. HEK-293T cells were co-transfected with the indicated plasmids (ΔC1-ΔC7 and FLAG-mGRIN2) and immunoprecipitated (IP) with the M2-FLAG antibody. Immunoprecipitates were immunoblotted (WB) with anti-DsRed (panel 1), anti-HA (panel 2), and M2-FLAG antibodies (panel 3), respectively. Whole cell lysates (WCL) were also immunoblotted with anti-DsRed antibody (panel 4), anti-HA antibody (panel 5), and M2-FLAG antibody (panel 6). Representative immunoblots from one of three independent experiments are shown.