TABLE 1.
Activity of eA1 G-H loop mutants in GBM cells
+ to +++, arbitrary degree of potentiation of eA1 and its substitution mutants activity. - to —, arbitrary degree of alteration of eA1 mutants activity. NA, not done.
| Mutant | EphA2 down-regulation | p-ERK down-regulation | p-Akt down-regulation |
|---|---|---|---|
| WT | ++ | ++ | + |
| F108A | ++ | - | + |
| Q109A | +++ | +++ | ++ |
| R110A | – | – | - |
| F111A | – | NA | NA |
| T112A | – | NA | NA |
| P113A | +++ | +++ | +++ |
| F114A | NA | NA | NA |
| T115A | +++ | +++ | +++ |
| L116A | – | NA | NA |
| G117A | +++ | +++ | +++ |
| K118A | ++ | NA | NA |
| E119A | – | NA | NA |
| F120A | – | — | – |
| K121A | ++ | - | – |
| E122A | +++ | +++ | +++ |
| G123A | – | – | + |