Skip to main content
. 2012 Mar 7;287(17):14301–14309. doi: 10.1074/jbc.M111.337642

FIGURE 1.

FIGURE 1.

GALNT7 is directly repressed by miR-214. A, sequence alignment of miR-214 with the wild-type and mutant (mut) 3′-UTR of GALNT7. B, the expression level of miR-214 in HeLa and C33A cells was significantly altered following transfection with either pri-miR-214 or ASO-miR-214 expression constructs as determined by qRT-PCR using U6 snRNA for normalization. C, the intensity of EGFP fluorescence in HeLa cells transfected with pri-miR-214 was decreased after 48 h and increased following transfection with ASO-miR-214. D, pri-miR-214 and ASO-miR-214 had no effect on the intensity of EGFP fluorescence in cells transfected with the 3′-UTR mutant vector. E and F, the mRNA (E) or protein (F) levels of GALNT7 in HeLa and C33A cells decreased or increased when compared with the control group when pri-miR-214 was overexpressed or blocked, respectively (*, p < 0.05, **, p < 0.005). Hsa, Homo sapiens; ptr, Pan troglodytes; Mml, Macaca mulatta; Oga, Otolemur garnettii; Cpo, Cavia porcellus.