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. 2012 Mar 2;287(17):13868–13876. doi: 10.1074/jbc.M111.334896

FIGURE 1.

FIGURE 1.

20-HETE is a novel activator for hTRPV1. A, left, currents evoked in response to 1 μm capsaicin, 10 μm 12-HPETE, and 10 μm 20-HETE in untransfected and hTRPV1-transfected HEK293 cells. A, right, summary of peak current densities evoked by 1 μm capsaicin (n = 38 cells of 13 individual experiments), 10 μm 12-HPETE (n = 10 cells of 4 experiments), and 10 μm 20-HETE (n = 35 cells of 14 experiments). B, effects of capsazepine on capsaicin and 20-HETE-evoked currents in hTRPV1-transfected HEK293 cells. 1–3 μm capsazepine decreases the response to 1 μm capsaicin (n = 6 cells of 3 experiments) and 10 μm 20-HETE (n = 5–6 cells of 3 experiments). C and D, concentration-peak current response curve for 0.01–10 μm capsaicin (C); responses normalized to response to 10 μm (n = 7 cells of 4 experiments) and 1–100 μm 20-HETE (D); responses normalized to the response to 100 μm (n = 7 cells of 4 experiments) in hTRPV1-transfected HEK293 cells. All summary data are shown as mean ± S.E. (error bars).