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. 2012 Mar 2;287(17):13868–13876. doi: 10.1074/jbc.M111.334896

FIGURE 2.

FIGURE 2.

20-Hete activates native murine TRPV1. A, representative traces of 1 μm capsaicin (upper) and 10 μm 20-HETE (lower)-evoked currents in voltage-clamped DRG neurons of WT and TRPV1 KO mice. Statistical significance was determined using unpaired Student's t test for n = 39 and 38 neurons of 10 and 9 mice, WT and KO, respectively, and is shown as ***, p < 0.001. B, Ca2+ imaging (Fluo-4 AM) of responses to 10–30 μm 20-HETE and 50 μm ionomycin in DRG neurons of WT and TRPV1 KO mice (left). Examples of fluorescence intensity (F/Fmax) measured against time are shown on the right. C, summary of F/F0 responses. Statistical significance was determined using unpaired Student's t test of n = 29, 28, and 16 neurons of 4, 4, and 3 mice, respectively, and shown as ***, p < 0.001). All summary data are shown as mean ± S.E. (error bars).