TABLE 1.

AFM-identified sequences bound by STAT3 in the pGAS-TA-Luc plasmid

Site	Distance from NotI site	Corresponding sequences
S100	85–110 bp	(S + 100) 5′-AAAACAAAACGAAACAAACAAACTA
		(S-100) 5′-CGCAACTGTTGGGAAGGGCGATCG
S333	320–350 bp	(S + 330) 5′-CGCCAAAAACATAAAGAAAG
		(S-330) 5′-GTTTTTCGCCCTTTGACGTTG
GAS	167–195 bp	(GAS) 5′- GTTTCCGGGAAAGCAGTAGGTTTCCGGGAAAG-3′
S+100		S + 100 mut#1 AAAACAGAACGAGACAGACAAACTA
mutantsa		S + 100 mut#2 AAGGCAGGACGAGGCAGGCAGGCTA

^a S+100 mutants were generated by substitution of A nucleotides within AT-rich hexanucleotides with G nucleotides. The positions of mutated bases are in bold and underlined.