Phylogenetic tree of VTC2-like proteins. Protein sequences homologous to C. reinhardtii VTC2 were used to build the phylogenetic tree as described under “Experimental Procedures.” Bootstrap values are shown below the branches. Bar, 0.2 amino acid substitutions per site. C. reinhardtii (Cre), Coccomyxa sp. C-169 (Coc_C169), Chlorella sp. NC64A (ChlNC64A), Volvox carteri f. nagariensis (Vca), O. lucimarinus (Ost9901), Ostreococcus sp. RCC809 (OstRCC809), M. pusilla (MicpuC2), Micromonas sp. RCC299 (MicpuN3), A. thaliana (Ath), Physcomitrella patens (Ppa), Ricinus communis (Rco), Oryza sativa (Osa), Selaginella moellendorffii (Selmo), D. melanogaster (Dme), C. elegans (Cel), and H. sapiens (Hsa).