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Acceptor specificity of C. reinhardtii recombinant VTC2. GDP-l-Gal and GDP-d-Glc consumption was measured by the HPLC assay described under “Experimental Procedures.” GDP-l-Gal or GDP-d-Glc were added to the reaction mixtures at a final concentration of 50 μm. The consumption of GDP-l-Gal (A) and GDP-d-Glc (B) was measured with a final enzyme concentration of 0.025 μg/ml (light gray bars) or 0.25 μg/ml (dark gray bars).
