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. 2012 Mar 5;287(17):13829–13839. doi: 10.1074/jbc.M112.344325

FIGURE 1.

FIGURE 1.

Characterization of M1- and M23-AQP4-expressing CHO-K1 and U87MG cells. A, confocal fluorescence microscopy (top) and TIRFM (bottom) of indicated cells immunostained for AQP4 using a C terminus anti-AQP4 antibody. B, SDS-PAGE (top) and Blue Native-PAGE (BN-PAGE, bottom) of cell homogenates. C, left, surface AQP4 expression quantified in live cells by immunostaining with 50 μg/ml rAb-58 for 1 h at 4 °C. Plasma membranes were stained with wheat germ agglutinin (WGA). Right, data are reported as the ratio of red (surface AQP4) to green (wheat germ agglutinin) fluorescence. (S.E., n = 5, *, p < 0.01).