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. 2012 Mar 5;287(17):13829–13839. doi: 10.1074/jbc.M112.344325

FIGURE 5.

FIGURE 5.

AQP4 assembly in OAPs is required for CDC. U87MG cells were transiently transfected with M1- or M23-AQP4, G28P-M23-AQP4 (OAP-disrupting mutation), or the indicated mixtures of M1- and M23-AQP4. A, TIRFM of transfected cells immunostained with anti-AQP4 antibody. B, CDC assay on transfected cells (S.E., n = 4, *, p < 0.001). C, fluorescence micrographs of transfected cells stained with 20 μg/ml rAb-58 (green) and anti-AQP4 antibody (red). Values for relative AQP4 cell surface expression are shown, normalized to that of M1-AQP4 (S.E., n = 3).