FIGURE 5.

Monocyte-to-macrophage differentiation is accompanied by altered expression of other matrix-related molecules. RT-PCR was performed for TSG-6 (A); HASs 1, 2, and 3 (B); and versican (C) mRNA levels at the indicated times after induction of differentiation with PMA. For each gene, mRNA levels in monocytes (0 h of PMA treatment) represent control levels. Values represent means ± S.D. (n = 3). No mRNA for HAS1could be detected at any time (N.D.). D, standard PCR was performed for bikunin and evaluated on an agarose gel. Replicate samples were analyzed for each time point. mRNA from HepG2 cells was used as a positive control. Error bars indicate S.D.