FIGURE 3.

DG_q is required for termination of light response. A, G_q protein levels in different treatments. cn,Gα_q⁹⁶¹,bw; P[hs::dGα_q] adults reared at 21 °C were exposed to 30 min of heat shock at 37 °C (30 min) or 1 h of heat shock at 37 °C (60 min) or no heat shock (no hs). All flies were collected and analyzed at 24 h after heat shock. INAD was used as the loading control. Note that the upper bands are nonspecific bands. Quantification of relative DG_q protein levels is shown on the right. B, ERG recordings showing termination kinetics of light responses in each treatment. G_q protein was induced as described in A. For all ERG traces, event markers represent 5-s orange light pulses, and the treatment conditions for each trace are marked. Quantification of the time required for half-recovery in each treatment, including wild-type controls, is shown on the right. C, representative ERG traces of cn,Gα_q⁹⁶¹,bw and cn,Gα_q⁹⁶¹,bw;gmr>G_q^H212A flies. Quantification of the time required for half-recovery in each treatment is shown on the right. Error bars indicate S.E.