FIGURE 5.
Lack of Gq leads to the impaired Arr2-Rh1 binding. A, assessment of deficits in Arr2-Rh1 binding. Dark-adapted flies were exposed to blue light for 30, 60, or 90 s, respectively. Supernatant (S) and membrane pellet (P) fractions of fly heads were subjected to Western blotting. D, kept in dark; B, blue light exposure. Quantification of Arr2-Rh1 binding is shown on the right. B, immunostaining of Arr2 and Rh1 in different genotypes and under different conditions. Sections were prepared as described under “Experimental Procedures.” Quantification of Arr2 in rhabdomeres is shown on the right. The averaged data from three independent sections are shown. Error bars, S.E.