FIGURE 7.

MiR-375 regulation on PGR. A, sequences of predicted miR-375 binding site in the 3′-UTR of PGR. B, target validation by luciferase assay in three different species. C, point mutation analysis of miR-375 seed binding sequence. The seed binding site (GAACAAA) was mutated into AACCAAA (to mimic the seed binding sequence in human). Cells were co-transfected with miR-375 precursor. D, Western blot analysis of PGR-A protein expression after a transfection of anti-miR-375 inhibitor or negative control in rhesus LLC-MK2 cells.