FIGURE 8.

Characterization of a long non-coding RNA, lncRNA-PGR-3p. A, genomic location of lncRNA-PGR-3p and primers used for RT-PCR. B, agarose gel analysis of RT-PCR amplification products. The amplification of genomic DNA was included as a positive control for primer design. RT-PCR with primers F2 and R2 revealed a novel transcript immediately downstream of the PGR gene locus. No transcript was detected from primers F1 and R1 or from primers F3 and R3, even though these primer sets could amplify genomic DNA efficiently.