FIGURE 7.

NMR spectra of L80F/L176F/V180F GCAP1 containing ¹³C-labeled myristoyl group. ¹³C-¹H heteronuclear multiple quantum correlation spectra of the ¹³C-labeled myristoyl group attached to unlabeled L80F/L176F/V180F GCAP1 in the Mg²⁺-bound activator state (A), Ca²⁺-bound inhibitory state (B), and denatured state in 8 m urea (C) are shown. Two-dimensional projections of ¹³C-edited NOESY spectra of the ¹³C-labeled myristoyl group attached to unlabeled L80F/L176F/V180F GCAP1 in the Mg²⁺-bound activator state (D), Ca²⁺-bound inhibitory state (E), and denatured state in 8 m urea (F) are shown. All NMR spectra were recorded at 800-MHz proton frequency. Sample conditions are described under “Materials and Methods.”