FIGURE 7.

Effect of RCAN1-1L overexpression on cell division and survival. A, dividing ST14A Cells. Cell number was measured using a Coulter Counter (Coulter Corp.). The results represent mean values of three independent experiments ± S.E. (error bars). After 72 h, the number of cells in the samples overexpressing RCAN1-1L was significantly (∼17%) lower than in controls as evaluated by Student's t test (p < 0.05). B, dividing ENStem cells. Cell number was measured using a Coulter Counter. The results represent mean values of three independent experiments ± S.E. After 72 h, the number of cells in the samples overexpressing RCAN1-1L was significantly (∼10%) lower than in controls, as evaluated by Student's t test (p < 0.05). C, differentiating ST14A cells. Cells were analyzed microscopically and counted manually. Only live differentiating cells were counted. The results represent the means of three independent experiments ± S.E. After 72 h, the number of cells in the samples overexpressing RCAN1-1L was significantly (∼17%) lower than in controls, as evaluated by Student's t test (p < 0.05). D, differentiating ENStem cells. Cells were grown and differentiated for 12 days (288 h). They were then analyzed microscopically and counted manually. Only live differentiating cells were counted. The results represent the means of three independent experiments ± S.E. The number of cells in the samples overexpressing RCAN1-1L was significantly (∼25%) lower than in controls, as evaluated by Student's t test (p < 0.05).