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Single-cell analysis of β subunit mRNA. Small-diameter (<25 μm) and large-diameter (>30 μm) DRG neurons were individually harvested, and the mRNA present in the cell lysates was reverse-transcribed and quantitatively measured by real-time PCR. The data are expressed as the number of mRNA copies present in each neuron. The data are the means ± S.E. of 74 small and 21 large neurons.
