FIGURE 3.

Caspase-2 inhibits tumor formation in athymic nude mice. A, representative mice with tumors circled in dashed outlines were derived from 1 × 10⁶ SV40/K-Ras-transformed Casp2^WT, Casp2^KO, Casp2^C320A, and Casp2^S139A MEFs injected (subcutaneously) in hind flanks of the mice and PBS as negative control. B, tumors were measured by caliper on day 14 after cell injection, and tumor volume was calculated by the equation V = (L × W²) × 0.5, where L is the length, and W is the width of the tumor. The data represent the average tumor volumes from nine mice for each cell line and are expressed as the means ± S.E. *p denotes significant difference compared with cell mass generated by wild-type cells.